产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 突触足蛋白抗体 |
| 别 名 | 9030217H17RIK; 9130229N11; 9330140I15Rik; AW046661; SYNAPTOPODIN; KIAA1029; SYNPO; SYNPO_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 信号转导 细胞凋亡 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, ) |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 Flow-Cyt=1μg /test （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 102kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Synaptopodin:601-700/903 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Synaptopodin is an actin-associated protein that may play a role in actin-based cell shape and motility. May be essential for the formation of spine apparatuses in spines of telencephalic neurons, involved in synaptic plasticity. The name synaptopodin derives from the protein's associations with postsynaptic densities and dendritic spines and with renal podocytes. Subunit: Interacts with BAIAP1. Interacts with actin (By similarity). Interacts (via PPxY motifs) with WWC1 (via WW domains). Subcellular Location: Cytoplasm, cytoskeleton (By similarity). Cell junction, tight junction (By similarity). Perikaryon (By similarity). Cell projection, dendritic spine (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Cell junction, synapse (By similarity). Note=Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses (By similarity). Tissue Specificity: Expressed in brain, namely in the olfactory bulb, cerebral cortex, striatum, and hippocampus, but not in the cerebellum. Also expressed in the podocytes of kidney glomeruli. In the hippocampus, mainly expressed in the principal cell layer of the dentate gyrus and Ammon's horn. Similarity: Belongs to the synaptopodin family. SWISS: Q8N3V7 Gene ID: 11346 Database links: Entrez Gene: 11346 Human Entrez Gene: 104027 Mouse Entrez Gene: 60324 Rat Omim: 608155 Human SwissProt: Q8N3V7 Human SwissProt: Q8CC35 Mouse SwissProt: Q9Z327 Rat Unigene: 435228 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. synaptopodin是一种与肌动蛋白微丝偶联的蛋白，其富含脯氨酸,为线状蛋白质，在动物体内表达于肾小球的足细胞和后脑的突触内，synaptopodin被认为是足细胞特异的分化成熟标志物。 |
| 产品图片 |  Sample: Cerebrum (Rat) Lysate at 40 ugPrimary: Anti-Synaptopodin (bs-3633R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 102 kD Observed band size: 76 kD  Sample: Brain (Mouse) Lysate at 40 ugPrimary: Anti-Synaptopodin (bs-3633R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 102 kD Observed band size: 102 kD  Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Synaptopodin) Polyclonal Antibody, Unconjugated (bs-3633R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Synaptopodin) Polyclonal Antibody, Unconjugated (bs-3633R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Positive control: RSC96Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody catalog number: bs-3633R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA |
