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磷酸化糖皮质激素调节激酶1抗体WB ELISA
产品名称:
磷酸化糖皮质激素调节激酶1抗体WB ELISA
英文名称:
Phospho-SGK1 (Ser422)
型号:
339611
产品库存
100
产品价格
电议

产品详情

 

中文名称 磷酸化糖皮质激素调节激酶1抗体
别    名 SGK1(Phospho Ser422); SGK1 (Phospho S422); Serine/threonine protein kinase SGK; Serine/threonine protein kinase Sgk1; Serine/threonine-protein kinase Sgk1; Serum and glucocorticoid regulated kinase; Serum/glucocorticoid regulated kinase 1; Serum/glucocorticoid regulated kinase; Serum/glucocorticoid-regulated kinase 1; SGK 1; SGK; SGK1; SGK1_HUMAN.  
产品类型 磷酸化抗体 
研究领域 免疫学  发育生物学  信号转导  干细胞  激酶和磷酸酶  细胞表面分子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Dog, Pig, Horse, Rabbit, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 49kDa
细胞定位 细胞核 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human SGK1 around the phosphorylation site of Ser422:GF(p-S)YA 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 SGK1 is a protein kinase that plays an important role in cellular stress response. SGK1 activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels of SGK1 and activity may contribute to conditions such as hypertension and diabetic nephropathy. This protein also mediates cell survival signals, as it has been shown to phosphorylate and negatively regulate the pro apoptotic FOXO3A protein. Ser 422 is a critical site on the protein and may be involved in its activation.

Function:
Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1.

Subunit:
Interacts with NEDD4 and NEDD4L.

Subcellular Location:
Isoform 2: Cell membrane.
Cytoplasm. Nucleus. Endoplasmic reticulum. Note=Nuclear, upon phosphorylation.

Tissue Specificity:
Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.

Post-translational modifications:
Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.

SWISS:
O00141

Gene ID:
6446

Database links:

Entrez Gene: 6446 Human

Entrez Gene: 20393 Mouse

Entrez Gene: 29517 Rat

Omim: 602958 Human

SwissProt: O00141 Human

SwissProt: Q9WVC6 Mouse

SwissProt: Q06226 Rat

Unigene: 510078 Human

Unigene: 28405 Mouse

Unigene: 4636 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti- Phospho-SGK1 (Ser422) (bs-3396R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample:
Lane 1: Pancreas (Mouse) Lysate at 40 ug
Lane 2: Kidney (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Pancreas (Rat) Lysate at 40 ug
Lane 5: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Phospho-SGK1 (Ser422) (bs-3396R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45-60 kD
Observed band size: 50 kD
Sample:
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-SGK1 (Ser422) (bs-3396R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-SGK1(Ser422) Polyclonal Antibody, Unconjugated(bs-3396R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-SGK1 antibody (bs-3396R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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