产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 髓系细胞触发受体2抗体 |
| 别 名 | TREM-2; TREM2a; TREM2b; TREM2c; Trggering receptor expressed on myeloid cells 2; Trggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; TREM2_HUMAN. |
| 研究领域 | 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 25kDa |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TREM2:1-100/230 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012] Function: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Subunit: Interacts with TYROBP/DAP12. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein (Potential). Isoform 2: Secreted (Potential). Isoform 3: Secreted (Potential). Tissue Specificity: Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord. DISEASE: Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q9NZC2 Gene ID: 54209 Database links: Entrez Gene: 54209 Human Entrez Gene: 83433 Mouse Omim: 605086 Human SwissProt: Q9NZC2 Human SwissProt: Q99NH8 Mouse Unigene: 435295 Human Unigene: 261623 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. TREM-2是近年来发现应用于诊断炎症性疾病的重要标记物,也是感染性疾病的预警指标,与细菌感染的严重程度相关。 TREM2属于免疫球蛋白超家族TREM,表达于白细胞的细胞膜表面受体,TREM-选择性地表达于中性粒细胞和部分单核细胞,它介导的信号传导通路在炎症的发生和级联放大中起重要作用。 |
| 产品图片 |  Sample:MCF-7(Human) Cell Lysate at 30 ug Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti- TREM2 (bs-2723R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 20 kD  Sample:MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-TREM2 (bs-2723R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD  Sample: Raji Cell lysate at 30ug;Primary: Anti-TREM2(bs-2723R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 23 kD Observed band size: 23 kD  Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TREM2) Polyclonal Antibody, Unconjugated (bs-2723R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (TREM2) Polyclonal Antibody, Unconjugated (bs-2723R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. |
