产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 整合素α1抗体 |
| 别 名 | CD 49a; CD49a; CD49 antigen-like family member A; CD49a; CD49a antigen; Integrin alpha-1; ITA1_HUMAN; Itga 1; ITGA1; Laminin and collagen receptor; Very late activation protein 1; VLA 1; VLA-1; VLA1. |
| 研究领域 | 细胞生物 免疫学 信号转导 干细胞 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Rat, (predicted: Mouse, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 128kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Integrin alpha 1:1001-1179/1179 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Alpha 1 integrin along with alpha 2, alpha L and alpha M has a unique inserted domain. Integrin alpha 1 is a receptor for laminin and collagen. The alpha1 subunit is also known as CD49a. CD49a associates with CD29 (beta 1 integrin), to form an alpha1 beta1 heterodimer, identified as the rat homologue to VLA1, which is involved in cellular adhesion to laminin and collagen. Function: Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Subunit: Heterodimer of an alpha and a beta subunit. Alpha-1 associates with beta-1. Interacts with RAB21. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain. SWISS: P56199 Gene ID: 3672 Database links: Entrez Gene: 3672 Human Entrez Gene: 109700 Mouse Entrez Gene: 25118 Rat Omim: 192968 Human SwissProt: P56199 Human SwissProt: Q3V3R4 Mouse SwissProt: P18614 Rat Unigene: 644352 Human Unigene: 482186 Mouse Unigene: 91044 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:Hela (Human) Cell Lysate at 40 ug Primary: Anti-Integrin alpha 1 (bs-2095R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 128 kD Observed band size: 128 kD  Sample:Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: 293T (Human) Cell Lysate at 30 ug Lane 3: U87MG (Human) Cell Lysate at 30 ug Lane 4: HepG2 (Human) Cell Lysate at 30 ug Lane 5: A549 (Human) Cell Lysate at 30 ug Primary: Anti-Integrin alpha 1 (bs-2095R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 150 kD Observed band size: 160 kD  Sample:A549 (Human) Cell Lysate at 40 ug Primary: Anti-Integrin alpha 1 (bs-2095R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 128 kD Observed band size: 128 kD  Blank control: U937(blue).Primary Antibody: Rabbit Anti- Integrin alpha 1 antibody(bs-2095R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-2095R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.  Blank control: RSC96 cells(blue).Primary Antibody: Rabbit Anti-Integrin alpha 1 antibody(bs-2095R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
