产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 线粒体内膜蛋白抗体 |
| 别 名 | Cell proliferation inducing protein 52; Cell proliferation-inducing gene 4/52 protein; HMP; IMMT; IMMT_HUMAN; Inner membrane protein mitochondrial; Inner mitochrondial membrane; MINOS2; Mitochondrial inner membrane organizing system 2; Mitochondrial inner membrane protein; Mitofilin; Motor protein; p87; p87/89; p89; pig4; PIG52; Proliferation-inducing gene 4; MICOS complex subunit MIC60. |
| 研究领域 | 心血管 信号转导 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Cow, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 68kDa |
| 细胞定位 | 细胞浆 细胞膜 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Mitofilin:241-340/758 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Mitochondria are the center of cellular energy production and essential metabolic reactions. As double membrane-bound organelles, mitochondria from different species, tissues, and metabolic states are highly polymorphic in nature, yet exhibit common structural features. The ultrastructural variations in mitochondrial architecture occur mainly due to the differences in the amount and shape of cristae. Abundant cristae are found in mitochondria from tissues where energy demand is high. Analysis of the human heart mitochondrial proteome shows that mitofilin is one of the most abundant mitochondrial proteins. It appears to play an important role in the maintenance of cristae morphology. Mitofilin was originally described as heart muscle protein (HMP) because of its high expression in the heart. Subunit: Interacts with OPA1, preferentially with the soluble OPA1 form. Component of the MINOS/MitOS complex, that includes IMMT, HSPA9 and CHCHD3 and associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2. Interacts with CHCHD6; the interaction is direct. Subcellular Location: Mitochondrion inner membrane. SWISS: Q16891 Gene ID: 10989 Database links: Entrez Gene: 508853 Cow Entrez Gene: 10989 Human Entrez Gene: 76614 Mouse Entrez Gene: 312444 Rat Omim: 600378 Human SwissProt: Q16891 Human SwissProt: Q8CAQ8 Mouse SwissProt: Q3KR86 Rat Unigene: 148559 Human Unigene: 235123 Mouse Unigene: 47124 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:A673(Human) Cell Lysate at 30 ug Primary: Anti-Mitofilin (bs-1824R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD  Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mitofilin) Polyclonal Antibody, Unconjugated (bs-1824R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mitofilin) Polyclonal Antibody, Unconjugated (bs-1824R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Human HepG2 cells fixed with 3.7% Formaldehyde in medium, blocking performed with 10% NGS in 1x PBS buffer + 0.1% Tween20. Incubated with Anti-Mitofilin/IMMT Polyclonal Antibody (bs-1824R) at a 1:10 dilution for 1hr at RT, followed by Anti-Rabbit secondary, Cy3 conjugate at a 1:300 dilution incubated at RT for 1hr. This image was kindly provided by an end-user. Human HepG2 cells fixed with 3.7% Formaldehyde in medium, blocking performed with 10% NGS in 1x PBS buffer + 0.1% Tween20. Incubated with Anti-Mitofilin/IMMT Polyclonal Antibody (bs-1824R) at a 1:10 dilution for 1hr at RT, followed by Anti-Rabbit secondary, Cy3 conjugate at a 1:300 dilution incubated at RT for 1hr. This image was kindly provided by an end-user. |
