产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 髓鞘相关糖蛋白CD57抗体 |
| 别 名 | 3-glucuronyltransferase 1; 3-glucuronyltransferase; B3GA1_HUMAN; B3GAT1; Beta 1 3 glucuronyltransferase 1; Beta-1; CD 57; CD57; CD57 antigen; Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT P; GlcAT-P; GLCATP; GlcUAT P; GlcUAT-P; GlcUATP; Glucuronosyltransferase P; HNK 1; HNK1; LEU 7; LEU7; LEU7 antigen; NK 1; NK1; UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase; UDP-GlcUA:glycoprotein beta-1. |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Rat, (predicted: Mouse, Chicken, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 38kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD57:21-120/334 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | CD57 recognizes a (Mr 100-110kDa) oligosaccharide antigenic determinant on myeloid cells and on a variety of polypeptides, lipids and chondroitan sulfate proteoglycans. This surface antigen is associated with myelin-associated glycoprotein (MAG). The CD57 antigen is present on 15-20% of normal peripheral blood mononuclear cells. It is expressed on a subset of natural killer cells (60%) and on a subset of T-lymphocytes. This carbohydrate is also present on N-CAM in the nervous system. Function: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group. Subunit: Homodimer (Potential). Subcellular Location: Golgi apparatus membrane; Single-pass type II membrane protein. Tissue Specificity: Mainly expressed in the brain. Similarity: Belongs to the glycosyltransferase 43 family. SWISS: Q9P2W7 Gene ID: 27087 Database links: Entrez Gene: 27087 Human Omim: 151290 Human SwissProt: Q9P2W7 Human Unigene: 381050 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD57是一种分子量为110KD的糖蛋白,它是自然杀伤细胞(NK)和杀伤细胞的特异性表面抗原。CD57表达于15-20%的外周血单核细胞，60%的NK活化细胞以及T细胞亚群。该抗体可以标记NK细胞，也可识别非淋巴组织的一些细胞。可用于NK细胞介导的细胞毒、NK细胞及T细胞亚群的功能等方面的研究，也可标记神经内分泌细胞及其肿瘤。 |
| 产品图片 |  Sample:Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-CD57 (bs-1635R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 63 kD  Sample:Brain (Mouse) Lysate at 40 ug Primary: Anti-CD57 (bs-1635R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 63 kD  Sample:Lung (Mouse) Lysate at 40 ug Lymph node (Mouse) Lysate at 40 ug Brain (Mouse) Lysate at 40 ug U-87MG (Human) Cell Lysate at 30 ug Primary: CD57(bs-1635R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 63 kD  Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD57) Polyclonal Antibody, Unconjugated (bs-1635R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: U2OS.Primary Antibody (green line): Rabbit Anti-CD57 antibody (bs-1635R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature.Acquisition of 20,000 events was performed. |
