产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 褪黑素受体1B抗体 |
| 别 名 | MT2; melatonin receptor type 1B; melatonin receptor; MEL1B; MEL-1B-R; Mtnr1b; MGC129286;MGC129287; MTNR-1B; Melatonin receptor 1B; MTR1B_HUMAN. |
| 研究领域 | 细胞生物 免疫学 神经生物学 生长因子和激素 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 40kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MTNR1B:201-300/362 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. Function: High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in retina and less in brain and hippocampus. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P49286 Gene ID: 4544 Database links: Entrez Gene: 4544 Human Omim: 600804 Human SwissProt: P49286 Human Unigene: 569039 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 有学者认为：MTNR1B是组成控制褪黑激素信号通路的一部分。 褪黑激素调解人体的昼夜节律，是通过白天和黑夜来控制睡眠和饮食的内部时钟。MTNR1B的进一步研究为高血糖和糖尿病与昼夜节律遭到破坏有直接关系这一观点提供了证据。 |
| 产品图片 |  Sample:Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Cerebellum (Mouse) Lysate at 40 ug Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 4: Cerebrum (Rat) Lysate at 40 ug Lane 5: Cerebellum (Rat) Lysate at 40 ug Lane 6: Jurkat (Human) Cell Lysate at 30 ug Lane 7: Hela (Human) Cell Lysate at 30 ug Lane 8: U87MG (Human) Cell Lysate at 30 ug Primary: Anti-MTNR1B (bs-0963R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/50-55 kD Observed band size: 60/50 kD  Sample:HepG2(Human) Cell Lysate at 30 ug Primary: Anti- MTNR1B (bs-0963R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTNR1B) Polyclonal Antibody, Unconjugated (bs-0963R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat retina tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MTNR1B Polyclonal Antibody, Unconjugated(bs-0963R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MTNR1B Polyclonal Antibody, Unconjugated(bs-0963R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
