产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 黑皮质素-1受体抗体 |
| 别 名 | MC1 Receptor; CMM5; MC1-R; MC-1R; MC1R; Melanocortin 1 receptor; Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor); Melanocortin receptor 1; Melanocyte-stimulating hormone receptor; Melanotropin receptor; MSH-R; MSHR; MSHR_HUMAN; SHEP2. |
| 研究领域 | 细胞生物 神经生物学 信号转导 细胞膜受体 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1µg/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 35kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse MC-1R:271-317/317 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This intronless gene encodes the receptor protein for melanocyte -stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation. Function: Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Subcellular Location: Cell membrane. Tissue Specificity: Melanocytes and corticoadrenal tissue. DISEASE: Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5) [MIM:613099]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q01727 Gene ID: 4157 Database links: Entrez Gene: 4157 Human Omim: 155555 Human SwissProt: Q01726 Human Unigene: 513829 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 类固醇受体（Steroid Receptors） |
| 产品图片 |  Sample:MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti-MC1 Receptor (bs-1419R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kD  Sample:Hela(Human) Cell Lysate at 30 ug Raji(Human) Cell Lysate at 30 ug Primary: Anti-MC1 Receptor (bs-1419R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kD  Tissue/cell: human melanoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MSHR Polyclonal Antibody, Unconjugated(bs-1419R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human melanoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MSHR Polyclonal Antibody, Unconjugated(bs-1419R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: 293T(blue).Primary Antibody: Rabbit Anti- MC1 Receptor/AF488 Conjugated antibody (bs-1419R-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-1419R-AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed. |
