产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 肿瘤转移相关蛋白1抗体 |
| 别 名 | Metastasis-associated 1; Metastasis associated 1; Metastasis associated gene 1; Metastasis associated gene 1 protein; Metastasis associated protein; Metastasis associated protein MTA 1; Metastasis associated protein MTA1; MTA 1; MTA1_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 81kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MTA1:601-715/715 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This protein was identified in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this protein has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70 kDa component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this protein suggests that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. Function: May be involved in the regulation of gene expression by covalent modification of histone proteins. Isoform Long is a corepressor of estrogen receptor (ER). Isoform Short binds to ER and sequesters it in the cytoplasm and enhances non-genomic responses of ER. Subunit: Component of the nucleosome-remodeling and histone-deacetylase multiprotein complex (NuRD). Interacts with HDAC1 and ITGB3BP/CENPR. Binds to CSNK1G2 in the cytoplasm. Subcellular Location: Cytoplasm and Nucleus. Tissue Specificity: Widely expressed. High expression in brain, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual. Similarity: Contains 1 BAH domain. Contains 1 ELM2 domain. Contains 1 GATA-type zinc finger. Contains 1 SANT domain. SWISS: Q13330 Gene ID: 9112 Database links: Entrez Gene: 9112 Human Entrez Gene: 116870 Mouse Entrez Gene: 64520 Rat Omim: 603526 Human SwissProt: Q13330 Human SwissProt: Q2KHS8 Mouse SwissProt: Q8K4B0 Mouse SwissProt: Q62599 Rat Unigene: 101448 Human Unigene: 525629 Human Unigene: 212577 Mouse Unigene: 5840 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 转录调节因子（Transcriptin Regulators） MTA1(Metastasis-associated 1)蛋白是一种蛋白复合物,它具有组蛋白脱乙酰基酶的活性,在组蛋白的脱乙酰基,染色质重塑,转录控制的过程中具有重要作用。 |
| 产品图片 |  Sample: Lung(Mouse) Lysate at 30 ugPrimary: Anti- MTA1 (bs-1412R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 81 kD Observed band size: 85 kD  Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTA1) Polyclonal Antibody, Unconjugated (bs-1412R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTA1) Polyclonal Antibody, Unconjugated (bs-1412R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MTA1 Polyclonal Antibody, Unconjugated(bs-1412R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: transplanted tumor of cervical carcinoma in nude mice; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MTA1 Polyclonal Antibody, Unconjugated(bs-1412R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
