产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 心肌缺血预处理正调节蛋白2抗体 |
| 别 名 | myocardial ischemic preconditioning upregulated protein 2; WDR26; PRO0852; CDW2; MIP2; CUL4 and DDB1 associated WDR protein 2; Myocardial ischemic preconditioning up regulated protein 2; myocardial ischemic preconditioning upregulated protein 2; WD repeat domain 26; WD repeat-containing protein 26; FLJ21016; WDR26_HUMAN. |
| 研究领域 | 心血管 细胞生物 信号转导 细胞周期蛋白 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Dog, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1µg/Test ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 72kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WDR26:101-200/514 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]. Function: May be involved in MAPK pathways. Subunit: Interacts with DDB1-CUL4A/B E3 ligase complexes. Subcellular Location: Cytoplasm. Tissue Specificity: Broadly expressed, with highest levels in heart and skeletal muscle. Similarity: Contains 1 CTLH domain. Contains 1 LisH domain. Contains 6 WD repeats. SWISS: Q9H7D7 Gene ID: 80232 Database links: Entrez Gene: 80232 Human Entrez Gene: 226757 Mouse Entrez Gene: 498301 Rat SwissProt: Q9H7D7 Human SwissProt: Q8C6G8 Mouse Unigene: 497873 Human Unigene: 289082 Mouse Unigene: 23078 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. WDR26可能具有通过MAPK信号途径调节细胞增殖的功能。 |
| 产品图片 |  Sample:Placenta (Mouse) Lysate at 40 ug Primary: Anti-WDR26 (bs-0932R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kD Observed band size: 70 kD  Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated(bs-0932R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated(bs-0932R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: MCF7; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (WDR26) Polyclonal Antibody, Unconjugated (bs-0932R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green). |
