产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 载脂蛋白H抗体 |
| 别 名 | Apolipoprotein H; Activated protein C binding protein; APC inhibitor; APOH; B2G1; beta 2 glycoprotein I; beta 2 glycoprotein I precursor; BG; OTTMUSP00000003033; APOH_HUMAN. |
| 研究领域 | 心血管 免疫学 合成与降解 糖蛋白 脂蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 36kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ApoH:281-345/345 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Apolipoprotein H is expressed by placental trophoblast cells at high levels. Although the normal physiological role is not known, the protein appears to act as a co-factor for the binding of autoantibodies to phospholipids to trophoblasts, which is a process involved in the pathogenesis of recurrent miscarriage. Function: Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. Subcellular Location: Secreted. Tissue Specificity: Expressed by the liver and secreted in plasma. Post-translational modifications: N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele. Similarity: Contains 4 Sushi (CCP/SCR) domains. SWISS: P02749 Gene ID: 350 Database links: Entrez Gene: 350 Human Entrez Gene: 287774 Rat Omim: 138700 Human SwissProt: P02749 Human SwissProt: P26644 Rat Unigene: 445358 Human Unigene: 1824 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 载脂蛋白H(apolipoprotein D,apoH)又称为β2糖蛋白Ⅰ(β2-glycoprotein,β2-GI)，是最初是在人血浆的高密度脂蛋白中被分离出来的。apoH在结构上与其他类型的载脂蛋白存在很大的差异,被归入脂肪促成素家族。 |
| 产品图片 |  Sample:HepG2 Cell (Human) Lysate at 30 ug Primary: Anti-APOH (bs- 1570R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35/50 kD  Sample:Lane 1: Liver (Mouse) Lysate at 40 ug Lane 2: Liver (Rat) Lysate at 40 ug Lane 3: Pancreas (Rat) Lysate at 40 ug Primary: Anti-APOH (bs-1570R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44-50 kD Observed band size: 47 kD  Sample:A549 Cell (Human) Lysate at 30 ug Primary: Anti-APOH (bs- 1570R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35/50 kD Tissue/cell: human laryngeal cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-APOH Polyclonal Antibody, Unconjugated(bs-1570R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
