产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 活化转录因子3抗体 |
| 别 名 | Activating transcription factor 3; ATF3deltaZip2; ATF3deltaZip2c; ATF3deltaZip3; cAMP dependent transcription factor ATF3; Cyclic AMP dependent transcription factor ATF3; Cyclic AMP-dependent transcription factor ATF-3; TF3_HUMAN; cAMP-dependent transcription factor ATF-3; cyclic AMP-dependent transcription factor ATF-3 isoform 1. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Chicken, Dog, Cow, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 20kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ATF3:191-181/181 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]. Function: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Subunit: Binds DNA as a homodimer or a heterodimer. Subcellular Location: Nucleus. Similarity: Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. SWISS: P18847 Gene ID: 467 Database links: Entrez Gene: 467 Human Entrez Gene: 11910 Mouse Entrez Gene: 25389 Rat Omim: 603148 Human SwissProt: P18847 Human SwissProt: Q60765 Mouse SwissProt: P29596 Rat Unigene: 460 Human Unigene: 2706 Mouse Unigene: 9664 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 转录调节因子（Transcriptin Regulators） 活化转录因子-3(属于activation transcription factor/cAMP responsive element binding protein家族)是真核细胞转录因子，同属于ATF/CREB家族。参与由cAMP或某些病毒蛋白质所诱导基因转录的调节。 转录激活因子3(activating transcription factor 3,ATF3)属含亮氨酸拉链结构的转录因子ATF/CREB家族成员，分子量20kDa,在大部分正常细胞中呈低浓度稳态表达。在正常组织中含量很低，在应激状态是表达增加，形成二聚体。ATF3是个应激早期快反应基因，体内外可被一系列损伤性应激信号快速诱导表达。ATF3不仅在应激反应中是个关键性的调控因子，还广泛参与了机体稳态维持、创伤愈合、细胞黏附、肿瘤形成、凋亡以及信号传导等生理和病理过程。ATF3与细胞生存和凋亡有关，但在不同的细胞状态下发挥不同甚至相反的作用。 |
| 产品图片 |  Sample: Embryo(Mouse) lysate 30ug;Primary: Anti-ATF3 (bs-0519R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; Predicted band size : 20kD Observed band size : 19kD  Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATF3 Polyclonal Antibody, Unconjugated(bs-0519R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATF3 Polyclonal Antibody, Unconjugated(bs-0519R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATF3 Polyclonal Antibody, Unconjugated(bs-0519R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATF3 Polyclonal Antibody, Unconjugated(bs-0519R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei |
