产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 巢蛋白1抗体 |
| 别 名 | Ent; Entactin 1; Entactin; NID 1; NID; NID-1; Nid1; Nidogen (enactin); Nidogen 1; Nidogen; Nidogen-1; NID1_HUMAN. |
| 研究领域 | 细胞生物 免疫学 神经生物学 信号转导 细胞周期蛋白 细胞粘附分子 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 133kDa |
| 细胞定位 | 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nidogen:201-300/1247 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Basement membranes are the earliest extracellular matrices produced during embryogenesis. They are synthesized and incorporated into the supramolecular architecture of several components, including laminins, Collagen IV, Nidogen and proteoglycans. Nidogen/Entactin, a sulfated glycoprotein, acts as a link between the extracellular matrix molecules Laminin 1 and Collagen Type IV, and thereby participates in the assembly of basement membranes. Nidogen is a highly conserved member of the Nidogen family, which also includes Nidogen-2. Nidogen-2 has a high level of N- and O-glycosylation, and it interacts with Collagens Type I and IV and Perlecan at a comparable level to Nidogen. Nidogen is synthesized and secreted in primary and established mesenchymal peritubular cells and myoepithelial cells, and it affects adhesion of peritubular cells in an autocrine manner. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation. Nidogen also cooperates with Laminin 1 to regulate b-casein expression. Function: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. Subunit: Interacts with FBLN1 and LGALS3BP. Interacts with PLXDC1. Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Post-translational modifications: N- and O-glycosylated. Similarity: Contains 6 EGF-like domains. Contains 1 NIDO domain. Contains 1 nidogen G2 beta-barrel domain. Contains 1 thyroglobulin type-1 domain. SWISS: P14543 Gene ID: 4811 Database links: Entrez Gene: 534319 Cow Entrez Gene: 4811 Human Entrez Gene: 18073 Mouse Entrez Gene: 25494 Rat Omim: 131390 Human SwissProt: P14543 Human SwissProt: P10493 Mouse SwissProt: P08460 Rat Unigene: 356624 Human Unigene: 4691 Mouse Unigene: 105658 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:Placenta (Mouse) Lysate at 40 ug Embryo (Mouse) Lysate at 40 ug Primary: Anti-Nidogen (bs-23656R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 133 kD Observed band size: 133 kD  Sample:Ovary (Mouse) Lysate at 40 ug Primary: Anti- Nidogen (bs-23656R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 133 kD Observed band size: 133 kD  Sample:Heart (Mouse) Lysate at 40 ug Primary: Anti- Nidogen (bs-23656R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 133 kD Observed band size: 133 kD  Sample:JEG-3(Human) Cell Lysate at 30 ug Primary: Anti-Nidogen (bs-23656R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 133 kD Observed band size: 133 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nidogen) Polyclonal Antibody, Unconjugated (bs-23656R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nidogen) Polyclonal Antibody, Unconjugated (bs-23656R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
