产品分类/PRODUCT CLASSIFICATION
| 中文名称 | Ki67蛋白抗体 |
| 别 名 | Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki-67; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_MOUSE. |
| 研究领域 | 肿瘤 细胞生物 信号转导 转录调节因子 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 358kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Ki-67:1231-1330/2938 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. Function: Thought to be required for maintaining cell proliferation. Subcellular Location: Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes. Similarity: Contains 1 FHA domain. SWISS: Q91VE6 Gene ID: 17345 Database links: Entrez Gene: 4288 Human Entrez Gene: 17345 Mouse Entrez Gene: 246042 Rat Omim: 176741 Human SwissProt: P46013 Human SwissProt: Q91VE6 Mouse SwissProt: Q5RJM0 Rat Unigene: 689823 Human Unigene: 80976 Human Unigene: 4078 Mouse Unigene: 233802 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 细胞增殖标志物(Proliferation Marker) Ki67与PCNA一样，为细胞增殖的一种标记，在细胞凋亡中S、G2 、M期均有表达，G0期缺如。 Ki-67增殖指数高低与许多肿瘤的分化程度、浸润、转移以及预后密切相关，因此被广泛作为各种恶性肿瘤的必检项目之一。 |
| 产品图片 |  Sample:MCF-7 Cell (human) Lysate at 40 ugPrimary: Anti-Ki67(bs-23103R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 358kD Observed band size: 358kD  Sample:NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-Ki-67 (bs-23103R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 358 kD Observed band size: 358 kD  Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (bs-23103R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (bs-23103R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse spieen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Proliferation related Ki 67 antigen ) Polyclonal Antibody, Unconjugated (bs-23103R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Blank control (blue line): mouse blood (fixed with 80% methanol (5 min at -20℃). Cells stained with Primary Antibody for 30 min at room temperature).Primary Antibody (green line): Rabbit Anti-Ki-67 antibody (bs-23103R),Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1μg /test. |
