产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 细胞色素c氧化酶IV亚型1抗体 |
| 别 名 | COX-IV; COX 4; COX 4I1; COX 4I2; COXIV Isoform 2; COX IV 1; COX IV-1; COX IV; COX4 2; COX4; COX4B; COX4I1; COX4I2; COX4L2; COXIV 2; COXIV; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome C Oxidase Subunit IV; Cytochrome c oxidase subunit IV isoform 1; Cytochrome c oxidase subunit IV isoform 2 (lung); dJ857M17.2; MGC72016; COX IV-1; Cox4a Cytochrome c oxidase polypeptide IV; COX41_HUMAN; COX4I1. |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 17kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human COX4:51-169/169 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008] Function: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Subcellular Location: Mitochondrion inner membrane. Post-translational modifications: Ubiquitous. Similarity: Belongs to the cytochrome c oxidase IV family. SWISS: P13073 Gene ID: 1327 Database links: Entrez Gene: 1327 Human Entrez Gene: 12857 Mouse Entrez Gene: 29445 Rat Entrez Gene: 281090 Cow Omim: 123864 Human SwissProt: P00423 Cow SwissProt: Q3SZS0 Cow SwissProt: P13073 Human SwissProt: P19783 Mouse SwissProt: P10888 Rat Unigene: 433419 Human Unigene: 386758 Mouse Unigene: 2528 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: kidney (Mouse) Lysate at 40 ugPrimary: Anti-COX4 (bs-10257R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1/5000 dilution Predicted band size: 17 kD Observed band size: 17 kD  Sample:heart (Mouse) Lysate at 40 ug Primary: Anti-COX4 (Bs-10257R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 17 kD  Sample: kidney (Mouse) Lysate at 40 ugPrimary: Anti-COX4 (bs-10257R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1/5000 dilution Predicted band size: 17 kD Observed band size: 17 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Polyclonal Antibody, Unconjugated (bs-10257R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-COX4 Polyclonal Antibody, Unconjugated(bs-10257R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Positive control: Hela cellsConcebtration: 5μg/10^6 cells Incubation conditions: Avoid light , 30 minutes on the ice. |
