丝氨酸/苏氨酸蛋白激酶3抗体-上海雅吉生物科技有限公司

英文名称

MST3/STK3

中文名称

丝氨酸/苏氨酸蛋白激酶3抗体

别名

Kinase responsive to stress 1; KRS1; Mammalian STE20 like protein kinase 2; Mammalian sterile 20-like 2; Mess1; MST; MST-2; MST2; Mst3; Serine/threonine kinase 3 (STE20 homolog, yeast); Serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine protein kinase 3; Serine/threonine protein kinase Krs1; STE20 like kinase MST2.

研究领域

细胞生物信号转导细胞凋亡转录调节因子激酶和磷酸酶

抗体来源

Rabbit

克隆类型

Polyclonal

交叉反应

Mouse, (predicted: Human, Rat, Chicken, Dog, Cow, Horse, Rabbit, )

产品应用

WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量

56kDa

细胞定位

细胞核细胞浆

性状

Liquid

浓度

1mg/ml

免疫原

KLH conjugated synthetic peptide derived from human STK3:101-200/491

亚型

IgG

纯化方法

affinity purified by Protein A

储存液

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMed

产品介绍

STK3 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation.

Subunit:
Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation. Interacts with and stabilizes SAV1. Interacts with RAF1, which prevents dimerization and phosphorylation. Interacts with RASSF1. Interacts (via SARAH domain) with isoform 1 of NEK2. Interacts with ESR1 only in the presence of SAV1. Interacts with PKB/AKT1. Forms a tripartite complex with MOBKL1B and STK38. Interacts with RASSF2 (via SARAH domain).

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.

Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Contains 1 SARAH domain.

SWISS:
Q9Y6E0

Gene ID:
8428

Database links:

Entrez Gene: 8428 Human

Entrez Gene: 223255 Mouse

Entrez Gene: 361092 Rat

Omim: 604984 Human

SwissProt: Q9Y6E0 Human

SwissProt: Q99KH8 Mouse

SwissProt: B0LT89 Rat

Unigene: 508514 Human

Unigene: 390756 Mouse

Unigene: 442081 Mouse

Unigene: 472866 Mouse

Unigene: 105397 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Sample:
Muslce (Mouse) Lysate at 40 ug
Fetal brain (Mouse) Lysate at 40 ug
Primary: Anti- MST3/STK3 (bs-7599R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- MST3/STK3 (bs-7599Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 52 kD

Sample:
Ovary (Mouse) Lysate at 40 ug
Primary: Anti- MST3/STK3 (bs-7599R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 53 kD

Sample:
Heart (Mouse) Lysate at 40 ug
Bladder (Mouse) Lysate at 40 ug
Primary: Anti- MST3/STK3 (bs-7599R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 53 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MST3 STK3) Polyclonal Antibody, Unconjugated (bs-7599R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-STK3/MST3 Polyclonal Antibody, Unconjugated(bs-7599R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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