产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 过氧化物酶体增殖物激活受体γ辅激活子1α+β抗体 |
| 别 名 | LEM6; Ligand effect modulator 6; PERC; Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; Peroxisome proliferative activated receptor, gamma, coactivator 1; Peroxisome proliferator activated receptor gamma coactivator 1 alpha; peroxisome proliferator-activated receptor gamma coactivator 1 beta; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; Peroxisome proliferator-activated receptor gamma coactivator 1-beta; peroxisome proliferator-activated receptor gamma, coactivator 1 beta; PGC 1 (alpha); PGC 1 alpha; PGC 1v; PGC-1(beta); PGC-1-alpha; PGC-1-beta; PGC-1-related estrogen receptor alpha coactivator; PGC1; PGC1(alpha); PGC1A; PGC1v; PPAR gamma coactivator 1 alpha; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPAR gamma coactivator-1beta; PPAR-gamma coactivator 1-alpha; PPAR-gamma coactivator 1-beta; PPARGC 1 alpha; PPARGC-1-alpha; PPARGC-1-beta; PPARGC1; PPARGC1A; Ppargc1b; PRGC1_HUMAN; PRGC2_HUMAN. |
| 研究领域 | 心血管 细胞生物 染色质和核信号 信号转导 糖尿病 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Rat, Pig, (predicted: Mouse, Dog, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 88/113kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PGC1 alpha + beta:151-250/798 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq]. Function: Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Subunit: Binds MYBBP1A, which inhibits transcriptional activation by this protein. Interacts with PRDM16. Interacts with LRPPRC. Homooligomer. Interacts with LPIN1. Subcellular Location: Nucleus. Tissue Specificity: Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects. Post-translational modifications: Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2. Similarity: Contains 1 RRM (RNA recognition motif) domain. SWISS: Q9UBK2 Gene ID: 10891 Database links: Entrez Gene: 10891 Human Entrez Gene: 133522 Human Entrez Gene: 170826 Mouse Entrez Gene: 19017 Mouse Entrez Gene: 291567 Rat Entrez Gene: 83516 Rat Omim: 604517 Human Omim: 608886 Human SwissProt: Q86YN6 Human SwissProt: Q9UBK2 Human SwissProt: 415302 Mouse SwissProt: O70343 Mouse SwissProt: Q8VHJ7 Mouse SwissProt: Q811R2 Rat SwissProt: Q9QYK2 Rat Unigene: 483816 Human Unigene: 527078 Human Unigene: 163382 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:A673(Human) Cell Lysate at 30 ug Primary: Anti-PGC1 alpha + beta (bs-7535R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88/113 kD Observed band size: 113 kD  Sample:Muscle (Pig) Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug Primary: Anti-PGC1 alpha + beta (bs-4064R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 88/113 kD Observed band size: 92 kD  Sample:Lane 1: Molt-4 (Human) Cell Lysate at 30 ug Lane 2: A431 (Human) Cell Lysate at 30 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: Hela (Human) Cell Lysate at 30 ug Lane 5: Jurkat (Human) Cell Lysate at 30 ug Lane 6: HL60 (Human) Cell Lysate at 30 ug Primary: Anti-PGC1 alpha + beta (bs-7535R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90/113 kD Observed band size: 113 kD  Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PGC1 alpha + beta) Polyclonal Antibody, Unconjugated (bs-7535R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PGC1 alpha+beta Polyclonal Antibody, Unconjugated(bs-7535R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control (Black line): Molt4 (Black).Primary Antibody (green line):Rabbit Anti-PGC1 alpha+beta antibody (bs-7535R) Dilution:1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
