产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 血管紧张素Ⅱ1A型受体抗体 |
| 别 名 | AGTR1; Agtr1a; AT1; AT1A; AT1AR; Type 1 angiotensin II receptor; AGTR1_HUMAN; AGTR1B; AT2R1; AT2R1B. |
| 研究领域 | 心血管 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 41kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from the middle of human Angiotensin II type 1A receptor:101-200/359 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | The Angiotensin II type 1 receptor (AT1) is the primary effector of Angiotensin II, a key regulator of blood pressure and fluid homeostasis. It is involved in pathogenesis of several cardiovascular diseases such as hypertension, cardiac hypertrophy and congestive heart failure. Angiotensin II interacts with two types of G-protein coupled membrane receptors, AT1 (type 1) and AT2 (type 2). AT1 has three isoforms in rodents: AT1A (359 aa), AT1B (359 aa), and AT1C (177 aa). Rat AT1's are predicted to contain seven transmembrane domains. The N-terminus is predicted to be extracellular, while the C-terminus is predicted to be cytoplasmic. AT1's are expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscle. Function: Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Subunit: Interacts with MAS1 (Probable). Interacts with ARRB1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Liver, lung, adrenal and adrenocortical adenomas. Post-translational modifications: C-terminal Ser or Thr residues may be phosphorylated. DISEASE: Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P30556 Gene ID: 185 Database links: Entrez Gene: 185 Human Entrez Gene: 24180 Rat Entrez Gene: 81638 Rat Omim: 106165 Human SwissProt: P30556 Human SwissProt: P34976 Rabbit SwissProt: P25095 Rat SwissProt: P29089 Rat Unigene: 477887 Human Unigene: 728754 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:Liver (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Adrenal gland (Rat) Lysate at 40 ug Primary: Anti-Angiotensin II type 1A receptor (bs-2132R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 56 kD  Sample:A549(Human) Cell Lysate at 430 ug Primary: Anti-Angiotensin II type 1A receptor (bs-2132R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41/51 kD  Sample:Lane 1: Liver (Mouse) Lysate at 40 ug Lane 2: Heart (Mouse) Lysate at 40 ug Lane 3: Placenta (Mouse) Lysate at 40 ug Lane 4: Kidney (Mouse) Lysate at 40 ug Lane 5: Embryo (Mouse) Lysate at 40 ug Lane 6: Cerebrum (Mouse) Lysate at 40 ug Lane 7: NIH/3T3 (Mouse) cell Lysate at 30 ug Lane 8: Liver (Rat) Lysate at 40 ug Lane 9: Heart (Rat) Lysate at 40 ug Lane 10: Kidney (Rat) Lysate at 40 ug Lane 11: Cerebrum (Rat) Lysate at 40 ug Lane 12: Embryo (Rat) Lysate at 40 ug Primary: Anti-Angiotensin II type 1A receptor (bs-2132R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 46 kD  Sample:Kidney (Mouse) Lysate at 40 ug Primary: Anti- Angiotensin II type 1A receptor (bs-2132R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 42 kD  Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (bs-2132R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: U87MG.Primary Antibody (green line): Rabbit Anti-Angiotensin II type 1A receptor antibody (bs-2132R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
