产品分类/PRODUCT CLASSIFICATION
| 中文名称 | CD28抗体 |
| 别 名 | CD 28; CD28 antigen; CD28 molecule; MGC138290; T cell antigen CD28; T cell specific surface glycoprotein; T cell specific surface glycoprotein CD28; Tp44. |
| 研究领域 | 细胞生物 免疫学 干细胞 细胞粘附分子 细胞表面分子 淋巴细胞 t-淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Chicken, Dog, Pig, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 25kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CD28:1-120/218 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation. Function: Possibly involved in T-cell activation. Subunit: Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P31041 Gene ID: 12487 Database links: Entrez Gene: 940 Human Entrez Gene: 12487 Mouse Omim: 186760 Human SwissProt: P10747 Human SwissProt: P31041 Mouse SwissProt: P42069 Rabbit Unigene: 591629 Human Unigene: 255003 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD28是免疫球蛋白超家族成员之一 ，为T细胞特异性由二硫键连接的同源二聚体表面糖蛋白，主要分布在外周血淋巴细胞。对迟发型超敏反应以及Tc介导的细胞毒作用都有免疫调节作用。 |
| 产品图片 |  Sample:Lane 1: Spleen (Mouse) Lysate at 40 ug Lane 2: Thymus (Mouse) Lysate at 40 ug Primary: Anti-CD28 (bs-1297R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD  Sample:Thymus (Rat) Lysate at 40 ug Primary: Anti-CD28 (bs-1297R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 44kD  Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD28) Polyclonal Antibody, Unconjugated (bs-1297R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated(bs-1297R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated(bs-1297R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Positive control: (mo)Splenocytes(2% Paraformaldehyde-fixed )Isotype Control Antibody Antibody: Rabbit IgG; Supplier: Cell Signaling Technology; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA Secondary Antibody Antibody: Goat anti-rabbit IgG-FITC; Supplier: Cell Signaling Technology; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody Supplier: Bioss; Supplier catalog number: bs-1297R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA |
