产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 丝氨酸蛋白酶抑制剂B7抗体 |
| 别 名 | SER-PINB7; MEGSIN; Mesangium predominant gene megsin; MGC120014; MGC120015; Serpin B7; Serpin peptidase inhibitor clade B (ovalbumin) member 7; TP55; SPB7_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 42kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Megsin:301-380/380 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Serpin B7 is a serine proteinase inhibitor of the ovalbumin like B clade of serpins. It was first discovered in a search for agents responsible for thrombopoiesis, and maturation of megakaryocytes. SerpinB7 was identified as a protein that increased acetylcholine esterase stimulation from bone barrow progenitor cells, and injection of SerpinB7 in mice induced a 40% increase in platelets compared to controls. Shortly thereafter, in a search for kidney specific genes, SerpinB7 was described as a protein specific to mesangial cells, and was named megsin to indicate the tissue specificity of the protein. Function: Might function as an inhibitor of Lys-specific proteases. Might influence the maturation of megakaryocytes via its action as a serpin. Subcellular Location: Cytoplasm. Tissue Specificity: Predominantly expressed in mesangial cells. Similarity: Belongs to the serpin family. Ov-serpin subfamily. SWISS: O75635 Gene ID: 8710 Database links: Entrez Gene: 8710 Human Omim: 603357 Human SwissProt: O75635 Human Unigene: 138202 Human Unigene: 742388 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. megsin是新近发现的蛋白质,属于丝氨酸蛋白酶抑制剂（Serpin）超家族，特异表达于肾小球系膜细胞,其表达异常在肾脏疾病进展中具有重要作用,megsin蛋白适量的表达，对维持人体正常的生理病理过程如凝血、纤维溶解、间质代谢、发育、血压、肿瘤和炎症等是很重要的。在系膜细胞增生和系膜基质增多明显的IgA肾病肾小球中，megsin的表达显著上调。 |
| 产品图片 |  Sample:Lane 1: Ear (Rat) Lysate at 40 ug Lane 2: Tongue (Rat) Lysate at 40 ug Primary: Anti-Megsin (bs-0815R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Megsin Polyclonal Antibody, Unconjugated(bs-0815R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
