产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 早期生长应答蛋白1抗体 |
| 别 名 | Egr-1; Early growth response 1; Krox-24; Ngf1; Ngfi; NGFI-A; zif-268; Egr1; A530045N19Rik; egr; Egr-1; ETR103; Krox-1; Krox-24; Krox24; NGF1-A; NGFI-A; NGFIA; TIS8; Zenk; Zfp-6; AT 225; AT225; Early growth response protein 1; G0S 30; G0S30; KROX 24; Krox 24 protein; KROX24; TIS 8; TIS8; Transcription factor ETR 103; Transcription factor ETR103; Transcription factor Zif 268; Transcription factor Zif268; Zfp 6; ZIF268; Zinc finger protein 225; ZNF 225; ZNF225; EGR1_HUMAN. |
| 研究领域 | 细胞生物 免疫学 信号转导 生长因子和激素 细胞周期蛋白 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2ug/test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 60kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Egr-1:401-500/453 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. [provided by RefSeq]. Function: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. Subcellular Location: Nucleus. Similarity: Belongs to the EGR C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers. SWISS: P18146 Gene ID: 1958 Database links: Entrez Gene: 1958 Human Entrez Gene: 13653 Mouse Omim: 128990 Human SwissProt: P18146 Human SwissProt: P08046 Mouse Unigene: 326035 Human Unigene: 708393 Human Unigene: 181959 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Egr-1为即刻早期基因家族中的一个成员,是一种含3个锌指结构的转录因子。有研究表明EGR-1在细胞生长分化中发挥重要作用，如在细胞周期G1-S期的转换过程中Egr-1被诱导表达。 |
| 产品图片 |  Sample:Lung (Mouse) Lysate at 40 ug Primary: Anti-Egr1 (bs-1076R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD  Sample:Liver (Rat) Lysate at 40 ug Primary: Anti- Egr1 (bs-1076R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Egr1) Polyclonal Antibody, Unconjugated (bs-1076R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Egr1 Polyclonal Antibody, Unconjugated(bs-1076R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat small intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Egr1 Polyclonal Antibody, Unconjugated(bs-1076R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Egr1 Antibody(bs-1076R) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange). |
