首页 > 抗体蛋白 > 抗体
alpha 1肾上腺素能受体A抗体
产品名称:
alpha 1肾上腺素能受体A抗体
英文名称:
ADRA1A
型号:
0600
产品库存
100
产品价格
电议

产品详情

 

中文名称 alpha 1肾上腺素能受体A抗体
别    名 ADA1A_HUMAN; Adrenergic alpha 1A receptor; Adrenergic alpha 1C receptor; Adrenergic alpha 1D receptor; alpha 1 Adrenergic Receptor; Alpha 1A adrenergic receptor; Alpha-1A adrenergic receptor; Alpha-1A adrenoreceptor; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c; ADRA1A; ADRA1C; Alpha 1A adrenoceptor; alpha-1A adrenergic receptor isoform 1; adrenergic, alpha-1A-, receptor variant 1; adrenergic, alpha-1A-, receptor variant 3; adrenergic, alpha-1A-, receptor variant 5; adrenergic, alpha-1A-, receptor variant 8; G protein coupled receptor; alpha-1A adrenoceptor; ADRA1L1; ALPHA1AAR.  

 

研究领域 细胞生物  神经生物学  细胞膜受体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 51kDa
细胞定位 细胞核 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Alpha-1A adrenergic receptor:201-300/466 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008].

Function:
This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.

Subunit:
Homo- and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes.

Subcellular Location:
Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.

Tissue Specificity:
Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.

Post-translational modifications:
C-terminal Ser or Thr residues may be phosphorylated.

Similarity:
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily.

SWISS:
P35348

Gene ID:
148

Database links:

Entrez Gene: 148 Human

Entrez Gene: 11549 Mouse

Entrez Gene: 29412 Rat

Omim: 104221 Human

SwissProt: P35348 Human

SwissProt: P97718 Mouse

SwissProt: P43140 Rat

Unigene: 52931 Human

Unigene: 709175 Human

Unigene: 57064 Mouse

Unigene: 9991 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

ADRA1肾上腺素能α1受体位于突触后,在血管平滑肌上,兴奋时可使血管收缩;
alpha 1-adrenergic receptor有兴奋效应也有抑制效应。肾上腺素能受体又可分为α和β两种。alpha 受体与儿茶酚胺结合后,主要是兴奋平滑肌,如血管收缩、子宫收缩和瞳孔开张肌收缩等;但也有抑制作用,如使小肠平滑肌舒张。β受体又可分为β1和β2两个亚型.
产品图片 Sample:
U937 Cell (Human) Lysate at 30 ug
Raji Cell (Human) Lysate at 30 ug
Primary: Anti-ADRA1A (bs-0600R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 55 kD
Sample:
kidney (Mouse) Lysate at 40 ug
Primary: Anti-ADRA1A (bs-0600R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 62 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-ADRA1A (bs-0600R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 62 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRA1/ADRA1B/alpha 1 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-0600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRA1/ADRA1B/alpha 1 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-0600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: U-87MG(blue).
Primary Antibody:Rabbit Anti-ADRA1A antibody(bs-0600R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (bs-0600R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

联系我们

TEL:021-34661275  点击拨打热线