产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 胰高血糖素样肽-1受体/GLP-1受体抗体 |
| 别 名 | Glucagon like peptide-1 receptor; GLP 1 R; GLP 1 receptor; GLP 1R; Glp1r; GLP; glp1r; Glucagon like peptide 1 receptor; RATGL1RCP; Glip;MGC138331; GLP-1-R; GLP1R_HUMAN; Glucagon-like peptide 1 receptor; GLP-1 receptor; GLP-1-R; GLP-1R; Precursor. |
| 研究领域 | 免疫学 生长因子和激素 糖尿病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 51kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GLP-1R:101-200/463 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | GLP1R is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.It has been suggested that this protein influences the feelings of satiety or hunger, sensation of glucose levels, control of glucagon sensitivity of islets, and non insulin-dependent diabetes mellitus. GLP1R is believed to be expressed in human pancreas,lung, brain, stomach, kidney and heart. ESTs have been isolated from skin and kidney libraries. Function: This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Subunit: May form homodimers and heterodimers with GIPR. Subcellular Location: Cell membrane; Multi-pass membrane protein. Post-translational modifications: N-glycosylation enhances cell surface expression and lengthens receptor half-life by preventing degradation in the ER. Similarity: Belongs to the G-protein coupled receptor 2 family. SWISS: O35659 Gene ID: 2740 Database links: Entrez Gene: 2740 Human Entrez Gene: 14652 Mouse Entrez Gene: 25051 Rat Omim: 138032 Human SwissProt: P43220 Human SwissProt: O35659 Mouse SwissProt: P32301 Rat Unigene: 389103 Human Unigene: 390969 Mouse Unigene: 11408 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. GLP-1R是GLP-1在体内作用的主要靶点,它是一个7次跨膜的G蛋白偶联受体,以cAMP为主要第二信使。GLP-1与GLP-1R结合过程是一个两结构域相互作用的过程,都与胰岛素分泌和糖代谢调节密切相关。 |
| 产品图片 |  Sample: Cerebrum(Rat) Lysate at 40 ugPrimary: Anti-GLP-1R (bs-1559R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD  Sample: Liver（Mouse） lysate at 30 ug;Primary: Anti- GLP-1R (bs-1559R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 51 kD Observed band size: 51 kD  Sample:Lane 1: Pancreas (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Primary: Anti-GLP-1R (bs-1559R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 50 kD  Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GLP-1R Polyclonal Antibody, Unconjugated(bs-1559R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GLP-1R Polyclonal Antibody, Unconjugated(bs-1559R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: SH-SY5Y.Primary Antibody (green line): Rabbit Anti-GLP-1R antibody (bs-1559R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.  Blank control: SH-SY5Y.Primary Antibody (green line): Rabbit Anti-GLP-1R antibody (bs-1559R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
