产品分类/PRODUCT CLASSIFICATION
| 英文名称 | laminin |
| 中文名称 | 层粘连蛋白抗体 |
| 别 名 | LAMA 1; LAMA; LAMA1; LAMB1; Laminin A chain; Laminin alpha 1; Laminin alpha 1 chain; Laminin B1 chain; Laminin subunit beta 1. |
| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 信号转导 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Cow, ) |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 198kDa |
| 细胞定位 | 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human laminin:901-1000/3075 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | laminins are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation; the laminin-type epidermal growth factor-like module occurs in tandem arrays; the domain contains 4 disulfide bonds (loops a-d) the first three resemble epidermal growth factor (EGF); the number of copies of this domain in the different forms of laminins is highly variable ranging from 3 up to 22 copies. Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Subcellular Location: Secreted > extracellular space > extracellular matrix > basement membrane. Tissue Specificity: Broadly expressed in: skin, heart, lung, and the reproductive tracts. Similarity: Contains 11 laminin EGF-like domains. Contains 1 laminin IV type A domain. Contains 1 laminin N-terminal domain. SWISS: P07942 Gene ID: 284217 Database links: Entrez Gene: 10319 Human Entrez Gene: 284217 Human Entrez Gene: 3908 Human Entrez Gene: 3912 Human Entrez Gene: 3913 Human Entrez Gene: 3915 Human Entrez Gene: 3918 Human Entrez Gene: 16772 Mouse Entrez Gene: 316758 Rat Omim: 150320 Human SwissProt: P07942 Human SwissProt: P24043 Human SwissProt: P25391 Human SwissProt: P55268 Human SwissProt: Q9Y6N6 Human SwissProt: P19137 Mouse SwissProt: Q9R0B6 Mouse Unigene: 201805 Human Unigene: 270364 Human Unigene: 302362 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 层粘连蛋白主要存在于基膜(basal lamina)结构中，是基膜所特有的非胶原糖蛋白。 层粘连蛋白在细胞发育过程中刺激细胞粘着、细胞运动。LN能够刺激胚胎中神经轴的生长,并促进成年动物的神经损伤后重生长和再生。如同纤粘连蛋白,细胞外的LN能够影响细胞的生长、迁移和分化。LN在原生殖细胞的迁移中起关键作用。 层粘连蛋白是细胞外基底膜的主要组成部分，分布于基底膜和细胞外基质内、成纤维细胞、内皮细胞和平滑肌细胞分泌层粘连蛋白。肿瘤的浸润和转移与其遭到破坏相关。该抗体可用于标记基底膜，有助于观察肿瘤周围基底膜的分布及其改变，间接提示该肿瘤的分化程度和浸润情况。 |
| 产品图片 |  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (bs-0821R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (bs-0821R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (bs-0821R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(bs-0821R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(bs-0821R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Transplantation tumor of In nude mice; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(bs-0821R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(bs-0821R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
