产品分类/PRODUCT CLASSIFICATION
| 规格 | 价格 | 库存 |
|---|---|---|
| 50ul | ￥ 980 | 200 |
| 100ul | ￥ 1680 | 200 |
| 200ul | ￥ 2500 | 200 |
| 中文名称 | 线粒体脱偶连蛋白2抗体 |
| 别 名 | Uncoupling Protein-2; UCP2; UCP 2; BMIQ4; Mitochondrial uncoupling protein 2; SLC25A8; UCPH; Uncoupling protein 2; Uncoupling protein 2 mitochondrial proton carrier; Solute carrier family 25 member 8; UCP2_MOUSE. |
| 研究领域 | 细胞生物 免疫学 染色质和核信号 糖尿病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Pig, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 34kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse UCP-2:201-309/309 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. UCP2 gene is expressed in many tissues, with the greatest expression in skeletal muscle. UCP2 is thought to play a role in non shivering thermogenesis, obesity and diabetes. Function: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Subunit: Acts as a dimer forming a proton channel (By similarity). Subcellular Location: Mitochondrion inner membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle. Similarity: Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats. SWISS: P70406 Gene ID: 7351 Database links: Entrez Gene: 7351 Human Entrez Gene: 54315 Rat Omim: 601693 Human SwissProt: P55851 Human SwissProt: P56500 Rat Unigene: 80658 Human Unigene: 13333 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. UCP-2又称解耦联蛋白 2 (UCP2 )是 1997年发现的一种新的解偶联蛋白 ,它是线粒体内膜载体家族的一员 。与其类似物UCP1相比 ,UCP2在棕色脂肪组织中含量较低 ,但在体内分布广泛。解耦联蛋白(UCP)具有使线粒体呼吸链解耦联作用,解耦联所释放的能量以热能的形式散发。在对UCP与产热关系的研究中,发现UCP2具有调节机体能量代谢的功能,并且可能是肥胖与2型糖尿病相关联的一个重要基因。 |
| 产品图片 |  Sample:Lane 1: Stomach (Mouse) Lysate at 40 ug Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 3: Du145 (Human) Cell Lysate at 30 ug Lane 4: A673 (Human) Cell Lysate at 30 ug Primary: Anti-UCP-2 (bs-1926R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD  Sample:Lane 1: Stomach (Rat) Lysate at 40 ug Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 3: SiHa (Human) Cell Lysate at 30 ug Lane 4: DU145 (Human) Cell Lysate at 30 ug Lane 5: RAW264.7 (Mouse) Cell Lysate at 30 ug Lane 6: Liver (Mouse) Lysate at 40 ug Primary: Anti-UCP-2 (bs-1926R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD  Sample:Stomach (Mouse) Lysate at 40 ug Primary: Anti- UCP-2 (bs-1926R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 32 kD  Sample:BV-2(Mouse) Cell Lysate at 30 ug Primary: Anti- UCP-2 (bs-1926R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 32 kD  Sample:Raw264.7(Mouse) Cell Lysate at 30 ug BV-2(Mouse) Cell Lysate at 30 ug Primary: Anti- UCP-2 (bs-1926R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kD  Sample:Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti- UCP-2 (bs-1926R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 33 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UCP-2) Polyclonal Antibody, Unconjugated (bs-1926R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control (blue line): Hela (blue).Primary Antibody (green line): Rabbit Anti-UCP-2 antibody (bs-1926R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
