产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 葡萄糖调节蛋白78/热休克蛋白70蛋白5抗体 |
| 别 名 | BIP; HSPA5; GRP-78; glucose regulated protein 78; 78 kDa glucose regulated protein; BIP; Endoplasmic reticulum lumenal Ca2+ binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; GRP78; Heat Shock 70kDa Protein 5; HSPA 5; HSPA-5; HSPA5; Immunoglobulin Heavy Chain Binding Protein; MIF2; GRP78_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 78kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GRP78:251-360/654 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. Function: Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Subcellular Location: Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. DISEASE: Note=Autoantigen in rheumatoid arthritis. Similarity: Belongs to the heat shock protein 70 family. SWISS: P11021 Gene ID: 3309 Database links: Entrez Gene: 3309 Human Entrez Gene: 14828 Mouse Entrez Gene: 25617 Rat Omim: 138120 Human SwissProt: P11021 Human SwissProt: P20029 Mouse SwissProt: P06761 Rat Unigene: 605502 Human Unigene: 743241 Human Unigene: 330160 Mouse Unigene: 470180 Mouse Unigene: 11088 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 葡萄糖调节蛋白78是热休克蛋白70家族的成员之一，又称：热休克蛋白70蛋白5,作为一种分子伴侣在蛋白质的折叠和转运过程及内质网应激反应中发挥重要作用.它在多种肿瘤组织中呈高表达. |
| 产品图片 |  Sample: Intestinal(Mouse) Lysate at 30ug;Primary: Anti-GRP78 (bs-1219R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution; Predicted band size : 78 kD Observed band size :78 kD  Sample:Lane 1: Spleen (Rat) Lysate at 40 ug Lane 2: Cerebrum (Rat) Lysate at 40 ug Lane 3: U-87MG (Human) Cell Lysate at 30 ug Lane 4: Hela (Human) Cell Lysate at 30 ug Lane 5: HepG2 (Human) Cell Lysate at 30 ug Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 ug Lane 7: A431 (Human) Cell Lysate at 30 ug Primary: Anti-GRP78 (bs-1219R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 74 kD  Sample:Placenta (Mouse) Lysate at 40 ug Primary: Anti- GRP78 (bs-1219R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 74 kD  Sample:Hyperpituitarism (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug Primary: Anti- GRP78 (bs-1219R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 74 kD  Sample:Liver(Rat) Lysate at 40 ug Primary: Anti- GRP78 (bs-1219R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 75 kD  Sample:U87mg(Human) Cell Lysate at 30 ug Primary: Anti- GRP78 (bs-1219R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 75 kD  Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP78) Polyclonal Antibody, Unconjugated (bs-1219R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GRP78 Polyclonal Antibody, Unconjugated(bs-1219R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
