产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 细胞外基质金属蛋白酶诱导因子抗体 |
| 别 名 | TCSF; Emmprin; 5A11 antigen; 5F7; Basigin (Ok blood group); Basigin; Blood brain barrier HT7 antigen; BSG; CD 147; CD147 antigen; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; M6 leukocyte activation antigen; neurothelin; OK; OK blood group; OK blood group antigen; TCSF; Tumor cell derived collagenase stimulatory factor; BASI_HUMAN. |
| 研究领域 | 肿瘤 免疫学 合成与降解 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Rabbit, Guinea Pig, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 40kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TCSF:301-385/38 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008 Function: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Subunit: Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms heterooligomers of isoform 2 and isoform 3. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM. Interacts with AJAP1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Melanosome. Tissue Specificity: Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. Post-translational modifications: N-glycosylated. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P35613 Gene ID: 682 Database links: Entrez Gene: 682 Human Entrez Gene: 12215 Mouse Entrez Gene: 25246 Rat Omim: 109480 Human SwissProt: P35613 Human SwissProt: P18572 Mouse SwissProt: P26453 Rat Unigene: 501293 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 合成与降解（Synthesis and Degradation） 主要由肿瘤细胞合成，刺激纤维母细胞产生MMP |
| 产品图片 |  Sample:Liver(Mouse) Lysate at 40 ug Placenta(Mouse) Lysate at 40 ug HepG2 Cell Lysate at 40 ug Primary: Anti-CD147 (bs-0684R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 60 kD  Sample:Brain (Mouse) Lysate at 40 ug Primary: Anti-CD147 (Bs-0684R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 50 kD  Sample:Lane 1: Liver (Mouse) Lysate at 40 ug Lane 2: Liver (Rat) Lysate at 40 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: U251 (Human) Cell Lysate at 30 ug Lane 5: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-CD147 (bs-0684R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50-60 kD Observed band size: 60 kD  Sample:Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-CD147 (Bs-0684R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 50 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD147) Polyclonal Antibody, Unconjugated (bs-0684R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD147) Polyclonal Antibody, Unconjugated (bs-0684R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD147/TCSF/Emmprin Polyclonal Antibody, Unconjugated(bs-0684R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  lank control (blue line): MCF7 (blue).Primary Antibody (green line):Rabbit Anti-CD147 antibody(bs-0684R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
