产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 热休克蛋白70抗体 |
| 别 名 | HSP70; HSP-70; HSP 70; Heat shock 70 kDa protein 1; heat shock 70kDa protein 1A; Heat shock 70kDa protein 1B; Heat shock induced protein; heat shock protein 70; HSP70 1; HSP70 2; HSP70.1; HSP72; HSPA1; HSPA1A; HSPA1B; XXbac BCX40G17.3 001; Heat shock 70 kDa protein 1A/1B; HSP71A_HUMAN; Hspa1a; Hsp70-1; HSP72; Hspa1; Hspa1b; Heat shock 70 kDa protein 1A/1B; shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Cow, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 Flow-Cyt=1ug/Test ICC=1:100 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 70kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HSP70:500-600/641 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]. Function: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Subunit: Component of the CatSper complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with TSC2. Interacts with IRAK1BP1. Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed. Interacts with DNAJC7. Interacts with CHCHD3. Subcellular Location: Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Tissue Specificity: HSPA1B is testis-specific. Similarity: Belongs to the heat shock protein 70 family. SWISS: P0DMV8 Gene ID: 3303 Database links: Entrez Gene: 281825 Cow Entrez Gene: 3303 Human Entrez Gene: 3304 Human Entrez Gene: 15511 Mouse Entrez Gene: 193740 Mouse Entrez Gene: 24472 Rat Entrez Gene: 294254 Rat Omim: 140550 Human Omim: 603012 Human SwissProt: Q27975 Cow SwissProt: P0DMV8 Human SwissProt: P0DMV9 Human SwissProt: P17879 Mouse SwissProt: Q61696 Mouse SwissProt: Q07439 Rat Unigene: 274402 Human Unigene: 719966 Human Unigene: 728810 Human Unigene: 1950 Rat Unigene: 228225 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. HSP-70是细胞受应激原刺激后诱导产生的一组应激蛋白，与肿瘤发生、增殖及分化有关。 环境和病生理性应激原均可导致肌体合成一组应急蛋白既热休克蛋白。许多热休克蛋白，包括HSP70家族成员，均参与蛋白的变性-复性、折叠-解折叠、运输-易位、活化-非活化和分泌等过程。 HSP70与类固醇受体、肌动蛋白、P53等蛋白密切相关。HSP70还参与热应激原、细胞毒药物和其他损伤所引起的应急反应，对机体起一定的保护作用。 |
| 产品图片 |  Sample:Lung (Mouse) Lysate at 40 ug Primary: Anti-HSP70 (bs-0244R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kD  Sample: Bladder (Mouse) Lysate at 40 ugPrimary: Anti-HSP70 (bs-0244R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kD  Sample: Lung (Mouse) Lysate at 40 ugPrimary: Anti-HSP70 (bs-0244R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kD  Sample:Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: Epididymis (Mouse) Lysate at 40 ug Lane 3: Seminal vesicle (Mouse) Lysate at 40 ug Lane 4: Liver (Mouse) Lysate at 40 ug Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 6: Testis (Rat) Lysate at 40 ug Lane 7: Spleen (Rat) Lysate at 40 ug Lane 8: Hela (Human) Cell Lysate at 30 ug Lane 9: Jurkat (Human) Cell Lysate at 30 ug Lane 10: HepG2 (Human) Cell Lysate at 30 ug Lane 11: A549 (Human) Cell Lysate at 30 ug Lane 12: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-HSP70 (bs-0244R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 68 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (bs-0244R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (bs-0244R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (bs-0244R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP70) polyclonal Antibody, Unconjugated (bs-0244R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control:A549.Primary Antibody (green line): Rabbit Anti-HSP70 antibody (bs-0244R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.  Blank control:A549.Primary Antibody (green line): Rabbit Anti-HSP70 antibody (bs-0244R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.  Blank control:293T.Primary Antibody (green line): Rabbit Anti-HSP70 antibody (bs-0244R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
