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FITC标记的CD4抗体
产品名称:
FITC标记的CD4抗体
英文名称:
CD4/FITC
型号:
Ys-0647R-FITC
产品库存
100
产品价格
电议

产品详情

研究领域 肿瘤  细胞生物  免疫学  干细胞  转录调节因子  细胞表面分子  t-淋巴细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, MACFA (predicted: Dog, Cow, Sheep, Guinea Pig, )
产品应用 Flow-Cyt=3ug/Test IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量  48kDa
细胞定位 细胞膜 
性    状    Lyophilized or Liquid
浓    度    1mg/ml
免 疫 原   KLH conjugated synthetic peptide derived from human CD4
亚    型    IgG
纯化方法  affinity purified by Protein A
储 存 液   0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍  background:
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010].
 
Function:
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
 
Subunit:
Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.
 
Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
 
Post-translational modifications:
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
 
Similarity:
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
 
Database links:
 
Entrez Gene: 920 Human
 
Entrez Gene: 12504 Mouse
 
Omim: 186940 Human
 
SwissProt: P06332 Mouse
 
SwissProt: P01730 Human
 
Unigene: 631659 Human
 
Unigene: 2209 Mouse
 
 
 
 
 
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
此抗体可识别55KDⅠ型单链穿膜糖蛋白。 CD4分子是存在于大多数辅助/诱导T细胞表面的59kDa的糖蛋白。正常淋巴组织中CD4的表达数量多于CD8,此抗体主要用于标记辅助/诱导T细胞,与CD8单抗联合使用对外周血淋巴细胞分型。 CD4抗原是HLA-II类分子和人类免疫缺陷病毒(HIV)-爱滋病的受体,在35-50%外周血淋巴细胞-辅助和诱导T细胞(Th/Ti)和70-80%人胸腺细胞上表达,在人的单核细胞表面也有低密度的表达。 CD4抗原有膜结合型和可溶性两种形式。Th/Ti可辅助Ig产生和T细胞毒T细胞的作用。
产品图片
Blank control: Mouse splen.
Primary Antibody (green line): Rabbit Anti-CD4 antibody (bs-0647R-FITC)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Dilution: 3μg /test.
Protocol
The cells incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

 

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