产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 核因子2相关因子2抗体 |
| 别 名 | Nuclear factor-E2 related factor2; Nrf-2; HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; Nrf 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid derived 2 like 2; NF2L2_HUMAN. |
| 研究领域 | 肿瘤 免疫学 染色质和核信号 合成与降解 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Dog, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:100-1000 ELISA=1:1000-5000 IHC-P=1:100-1000 IHC-F=1:100-1000 ICC=1:100 IF=1:100-1000 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 68kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nrf2:401-500/605 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Function: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Subunit: Heterodimer. May bind DNA with an unknown protein. Interacts with KEAP1. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1. Subcellular Location: Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. Tissue Specificity: Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle. Post-translational modifications: Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. Similarity: Belongs to the bZIP family. CNC subfamily. Contains 1 bZIP domain. SWISS: Q16236 Gene ID: 4780 Database links: Entrez Gene: 4780 Human Omim: 600492 Human SwissProt: Q16236 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 转录调节因子（Transcriptin Regulators） 核细胞系因子2相关因子Nrf2，是一种对氧化还原反应敏感的转录因子，可以通过正向促进基因的表达，来转录翻译产生一些抗氧化物，行使着异化解毒的酶类和药物的高效外排泵作用。 有学者认为：核因子2相关因子2蛋白在保护细胞免受环境毒性物质影响方面发挥着极其重要的作用，它指导某些基因促进细胞的防御工事，同时也刺激某些重要解毒酶对毒性物质，如黄曲霉素的解毒和排毒作用。 |
| 产品图片 |  Sample:Lane 1: Kidney (Mouse) Lysate at 40 ug Lane 2: Liver (Mouse) Lysate at 40 ug Lane 3: Liver ((Rat) Lysate at 40 ug Lane 4: SiHa (Human) Cell Lysate at 30 ug Lane 5: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-Nrf2 (bs-1074R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110’68 kD Observed band size: 110 kD  Sample:HepG2(Human) Cell Lysate at 40 ug MCF-7(Human) Cell Lysate at 40 ug Primary: Anti-Nrf2(bs-1074R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 68kD Observed band size: 68kD  Sample:A549 (Human)Cell Lysate at 40 ugPrimary: Anti-Nrf2(bs-1074R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 68kD Observed band size: 68kD Sample: Brain(Rat) lysate at 30ug; Brain(Mouse) lysate at 30ug; Primary: Anti-Nrf2 (bs-1074R) at 1:200 dilution Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution Predicted band size : 66kD Observed band size : 66kD Primary: Anti-Nrf2 (bs-1074R) at 1:200 Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 Predicted band size : 66kD Observed band size : 66kD  Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (bs-1074R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nrf2 Polyclonal Antibody, Unconjugated(bs-1074R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Nrf2)) polyclonal Antibody, Unconjugated (bs-1074R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. |
