DMEM/F12+Insulin 0.005 mg/ml+ Transferrin 0.01 mg/ml Sodium selenite +30 nM Hydrocortisone +10 nM β-estradiol +10 nM HEPES 10 mM +L-glutamine +2 mM (in addition to that in the base medium)+ FBS 10%
HITES medium supplemented with 2% fetal bovine serum HITES medium with 2% fetal bovine serum is formulated at the ATCC as follows: Dulbecco's medium : Ham's F12, 50:50 mix (ATCC30-2006) Insulin 0.005 mg/ml Transferrin 0.01 mg/ml Sodium selenite 30 nM Hydrocortisone 10 nM β-estradiol 10 nM HEPES 10 mM L glutamine (ATCC302214) 2 mM (in addition to that in the base medium) Fetal bovine serum (ATCC 30-2020) 2%
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