作者列表：Mai Yini, Sun Peng, Suo Yujing, Li Huawei, Han Weijuan, Diao Songfeng, Wang Liyuan, Yuan Jiaying, Wang Yiru, Ye Lingshuai, Zhang Yue, Li Fangdong, Fu Jianmin

期刊：Frontiers in Plant Science

影响因子：5.6

发表时间：2023-2-22

DOI:10.3389/fpls.2023.1046235

Abstract

Dioecy system is an important strategy for maintaining genetic diversity. The transcription factor MeGI, contributes to dioecy by promoting gynoecium development in Diospyros lotus ａnd D. kaki. However, the function of MeGI in D. oleifera has not been identified. In this study, we confirmed that MeGI, cloned from D. oleifera, repressed the androecium development in Arabidopsis thaliana. Subsequently, chromatin immunoprecipitation-sequencing （ChIP-seq）, DNA affinity purification-sequencing （DAP-seq）, ａnd RNA-seq were used to uncover the gene expression response to MeGI. The results showed that the genes upregulated ａnd downregulated in response to MeGI were mainly enriched in the circadian rhythm-related ａnd flavonoid biosynthetic pathways, respectively. Additionally, the WRKY DNA-binding protein 28 （WRKY28） gene, which was detected by ChIP-seq, DAP-seq, ａnd RNA-seq, was emphasized. WRKY28 has been reported to inhibit salicylic acid （SA） biosynthesis ａnd was upregulated in MeGI-overexpressing A. thaliana flowers, suggesting that MeGI represses the SA level by increasing the expression level of WRKY28. This was confirmed that SA level was lower in D. oleifera female floral buds than male. Overall, our findings indicate that the MeGI mediates its sex control function in D. oleifera mainly by regulating genes in the circadian rhythm, SA biosynthetic, ａnd flavonoid biosynthetic pathways.