作者列表：Shulong Zhang, Yijun Zhao, Li Geng, Feihong Song, Li Feng, Jun Jiang, Qianqian Cai, Fei Fan

发表时间：2026-2-24

期刊：ONCOLOGY RESEARCH

影响因子：4.1

DOI:10.1038/s41419-021-04210-9

主要研究成果：Abstract

Background

Hepatocellular carcinoma （HCC） is a highly lethal malignancy driven by both intrinsic oncogenic pathways ａnd immune microenvironmental regulation. Emerging evidence suggests that DNASE1L3 may influence tumor biology ａnd immune responses; however, its specific roles in HCC progression ａnd macrophage-mediated regulation remain unclear. This study aimed to elucidate the biological functions of DNASE1L3 in HCC ａnd to determine how it modulates tumor behavior ａnd immune interactions.

 

Methods

Bioinformatics analyses of the GSE41804 ａnd Cancer Genome Atlas-Liver Hepatocellular Carcinoma （TCGA-LIHC） datasets were used to identify hub genes. Functional assays assessed the impact of DNASE1L3 on HCC cell proliferation, migration, invasion, ａnd cell cycle progression. The effects of DNASE1L3 on macrophage polarization ａnd the Wnt/β-catenin signaling pathway were examined using a co-culture system. An HCC organoid model was established to further validate its regulatory function.