期刊：Journal of Healthcare Engineering

作者列表：Wenting Zhang, Xin Wang, Jing Li, Mingyuan Xu, Xiaolu Ren, Huiying Liu, Lu Xu, Jun Shao

发表时间：2021-12-3

影响因子：2.682

DOI:10.1155/2021/3433615

主要研究成果：Abstract

Osteoarthritis （OA） is a pervasive degenerative joint disease affecting companion animals, characterized by chronic inflammation ａnd cartilage degradation. However, the effectiveness of chondroitin sulfate （CS） in treating OA in dogs ａnd cats remains controversial. This study aimed to determine the therapeutic effects ａnd molecular mechanisms of CS on lipopolysaccharide （LPS）-induced inflammation in feline ａnd canine articular chondrocytes （FAC ａnd CAC） at the cellular level in vitro. Our findings demonstrated that CS treatment （800 µg/mL） significantly enhanced cell viability ａnd reduced oxidative stress in FAC ａnd CAC, as evidenced by decreased levels of reactive oxygen species ａnd increased activities of antioxidant enzymes. Furthermore, CS treatment effectively suppressed LPS-induced secretion of pro-inflammatory cytokines, including interleukin-1, tumor necrosis factor-α, interleukin-8, interleukin-10, ａnd matrix metalloproteinases-3, ａnd reduced apoptosis, as confirmed by fluorescence staining ａnd flow cytometry. Transcriptomic analysis revealed that CS upregulated neurotrophic signaling pathways, promoting cell survival ａnd proliferation. Metabolomic analysis indicated that CS treatment upregulated metabolites associated with glycerophospholipid ａnd purine metabolism, suggesting enhanced membrane integrity ａnd energy metabolism. Conversely, pathways involved in protein catabolism ａnd arachidonic acid metabolism were downregulated, indicating a reduction in inflammatory mediators. Collectively, these findings elucidate the multifaceted role of CS in modulating chondrocyte metabolism ａnd inflammatory responses, highlighting its potential to alleviate OA.