文章标题：Sub-minimum inhibitory concentrations of Rifampicin Attenuate Methicillin-resistant Staphylococcus aureus Virulence by Suppressing SaeRS Two-Component System and Arginine metabolism-related pathways
作者列表：Yanghua Xiao, Yanlei Xu, Weihua Han, Bingjie Wang, Fangyou Yu
影响因子：4.6
期刊：INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
发表时间：2025-2-3
DOI:10.1016/j.ijantimicag.2025.107459
文献主题：
Abstract
Objectives
Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant threat to global public health, prompting the exploration of alternative strategies to mitigate its virulence. This study investigates the impact of subminimum inhibitory concentrations (sub-MICs) of rifampicin on MRSA virulence, aiming to provide insights for optimizing antibiotic treatment strategies.
Methods
Enzyme-linked immunosorbent assay and western blot analysis were used to assess α-hemolysin expression. Transcriptomic sequencing and RT-qPCR analyzed gene expression changes in MRSA treated with sub-MICs of rifampicin. Mutant strains (ΔsaeR and ΔargGH) were constructed to validate the roles of the SaeRS system and arginine metabolism. Thermal shift assays evaluated the interaction between L-arginine and SaeR protein. In vivo murine models and Galleria mellonella infection models were used to assess the anti-virulence effects of rifampicin.
